Ferrous Ion Transport across Chloroplast Inner Envelope Membranes
نویسندگان
چکیده
منابع مشابه
Ferrous ion transport across chloroplast inner envelope membranes.
The initial rate of Fe(2+) movement across the inner envelope membrane of pea (Pisum sativum) chloroplasts was directly measured by stopped-flow spectrofluorometry using membrane vesicles loaded with the Fe(2+)-sensitive fluorophore, Phen Green SK. The rate of Fe(2+) transport was rapid, coming to equilibrium within 3s. The maximal rate and concentration dependence of Fe(2+) transport in predom...
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The glycolate/glycerate transporter of spinach (Spinacia oleracea L.) chloroplast inner envelope membranes was solubilized by treatment of the membranes with sodium cholate. Mixtures of the cholate extracts and soy asolectin were subjected to gel filtration to remove the detergent. The reconstituted vesicles were frozen, thawed, and sonicated in a buffer that contained 10 millimolar d-glycerate...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 2002
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.010858